Isolation of a retinal pigment epithelial cell-derived fraction which promotes Muller cell proliferation

Department

Natural Sciences

Document Type

Article

Publication Source

Developmental Brain Research

Publication Date

2000-04-14

Volume

120

Issue

2

First Page

267

Last Page

271

Abstract

Retinal pigment epithelial cells (RPE) secrete a factor(s) in vitro which promotes Muller cell proliferation. To begin the isolation of the active factor, medium conditioned by cultured RPE (RPE-CM) was fractionated by fast protein liquid chromatography. The fractions were tested in an assay for Muller cell proliferation and it was found that one fraction contained biological activity comparable to that of complete RPE-CM. Gel electrophoresis demonstrated that this fraction consisted of proteins with approximate molecular masses of 35-88 kDa. An affinity assay revealed that only an 88-kDa protein in the RPE-CM binds to Muller cells. This protein was also present in the active fraction; therefore, it is the most likely source of the mitogenic activity of RPE-CM. Copyright (C) 2000 Elsevier Science B.V.

Keywords

Affinity assay, Fast protein liquid chromatography, Growth factor, Proliferative vitreoretinopathy (PVR), Rat

DOI

10.1016/S0165-3806(00)00017-1

https://doi.org/10.1016/S0165-3806(00)00017-1

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