Isolation of a retinal pigment epithelial cell-derived fraction which promotes Muller cell proliferation
Developmental Brain Research
Retinal pigment epithelial cells (RPE) secrete a factor(s) in vitro which promotes Muller cell proliferation. To begin the isolation of the active factor, medium conditioned by cultured RPE (RPE-CM) was fractionated by fast protein liquid chromatography. The fractions were tested in an assay for Muller cell proliferation and it was found that one fraction contained biological activity comparable to that of complete RPE-CM. Gel electrophoresis demonstrated that this fraction consisted of proteins with approximate molecular masses of 35-88 kDa. An affinity assay revealed that only an 88-kDa protein in the RPE-CM binds to Muller cells. This protein was also present in the active fraction; therefore, it is the most likely source of the mitogenic activity of RPE-CM. Copyright (C) 2000 Elsevier Science B.V.
Affinity assay, Fast protein liquid chromatography, Growth factor, Proliferative vitreoretinopathy (PVR), Rat
Jaynes, C. David and Turner, James E., "Isolation of a retinal pigment epithelial cell-derived fraction which promotes Muller cell proliferation" (2000). Articles & Book Chapters. 410.